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PUC19 Vector Cloning

This post categorized under Vector and posted on March 8th, 2020.

PUC19 is a commonly used plasmid cloning vector in E.coli. The molecule is a small double-stranded circle 2686 base pairs in graphicgth and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases. pUC19 gehrt wie auch pUC18 zu einer Reihe im Labor von Joachim Messing gentechnologisch hergestellter Klonierungsvektoren. Diese bakterielgraphic Plasmide gehren zu den am hufigsten verwendeten Vektoren zur Klonierung im Bakterium Escherichia coli. Damit haben sie in der biologischen Forschung und Gentechnik eine groe Bedeutung. pUC19 Standard E. coli vector with a multiple cloning site (MCS) for DNA cloning. The MCS is reversed in pUC18.

PUC19 is 2686bp Plasmid. pUC19 carries antibiotic resistance gene ampicillin. pUC19 has N-terminal fragment of -galactosidase gene of (LacZ) E.coli. Multiple Cloning Site (MCS) Multicloning pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle 2686 base pairs in graphicgth and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases (1). pUC19 is one of a series of plasmid cloning vectors created by Joachim Messing and co-workers. The designation pUC is derived from the clgraphicical p prefix (denoting plasmid) and the abbreviation for the University of California where early work on the plasmid series had been conducted.

PUC19 Standard E. coli vector with a multiple cloning site (MCS) for DNA cloning. pUC19 is a small high-copy number E. coli plasmid cloning vector of which multiple cloning sites as shown below. The molecule is a small double-stranded circle 2686 base pairs in graphicgth. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa) which allows for bluewhite colony screening (i.e. a-complementation) as well as a pUC origin of replication. Cloning Vector pUC19 Product Information Sheet V33202 MoBiTec GmbH Germany Phone 49 551 70722 0 Fax 49 551 70722 22 E-Mail infomobitec.com www.mobitec.com Revised December 2013 1 SUMMARY Product pUC19 high copy cloning vector for replication in E.coli suitable for blue-white screening technique. Description This PCR Cloning Kit contains an optimized 2X Cloning Master Mix with a proprietary ligation enhancer and a linearized vector that uses a novel mechanism for background colony suppression to give a low background. It allows simple and quick cloning of any PCR amplicon whether the amplification reactions are performed with proofreading DNA polymerases such as Q5 or Phusion which produce

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PUC19 Vector Cloning: Vector Construction And Triclosan Selection A The Bla Gene In Puc Which Confersfig

Vector Construction And Triclosan Selection A The Bla Gene In Puc Which Confersfig

Vector construction and triclosan selection. (A) The bla gene in pUC19 which confers ampicillin resistance was replaced with fabI and its promoter [more]

PUC19 Vector Cloning: Cloning Of The Ctndot Left Junction The Integrated Insertional Shuttle Vector Pnlyfig

Cloning Of The Ctndot Left Junction The Integrated Insertional Shuttle Vector Pnlyfig

Cloning of the CTnDOT left junction. The integrated insertional shuttle vector pNLY3 containing 368 bp of traQ pNLY3traQ is shown integrated into t [more]

PUC19 Vector Cloning: Cointegrate Plasmid Pjaz And Subsequent Construction Of Pjaz By Cloning The Kbfig

Cointegrate Plasmid Pjaz And Subsequent Construction Of Pjaz By Cloning The Kbfig

SMRT Sequencing of Long Tandem Nucleotide Repeats in SCA10 Reveals Unique Insight of Repeat Expansion Structure.pdf Available via l [more]

PUC19 Vector Cloning: Restriction Enzymes Puc Vector Bp Length Two Restriction Sites Hindiii Sali Want Cl Q

Restriction Enzymes Puc Vector Bp Length Two Restriction Sites Hindiii Sali Want Cl Q

with EcoRI and cloned it into a unique EcoRI restriction site in the vector. a) How can you use the EcoRI restriction enzyme to tel [more]

PUC19 Vector Cloning: Assays Of Ivec Activities A A Scheme Of In Vivo Cloning By Assembly Of Two Dnafig

Assays Of Ivec Activities A A Scheme Of In Vivo Cloning By Assembly Of Two Dnafig

The development and functional activities of B lymphocytes critically depend on their migratory capacity. Both in vitro and in vivo [more]

PUC19 Vector Cloning: Clonaci N Y Vectores Plasm Dicos

Clonaci N Y Vectores Plasm Dicos

1771497-1504 1995). Esto se debe a que los vectores plasm dicos en multicopia usados compiten con la actividad metab olica de la ba [more]

PUC19 Vector Cloning: Microbes And The Tools Of Genetic Engineering

Microbes And The Tools Of Genetic Engineering

Each DNA strand contains thousands of genes. Gene cloning is the process of replicating a specific set of genes from a strand of DN [more]

PUC19 Vector Cloning: Problem Restriction Enzymes Points Parts Common Feature Plasmids Used Molecular Clo Q

Problem Restriction Enzymes Points Parts Common Feature Plasmids Used Molecular Clo Q

This vector describes how to vectoryze restriction enzyme digests on circular plasmid DNA. Emphasis is placed on predicting the num [more]

PUC19 Vector Cloning: Features Vectors Plasmids Must Selectable Marker Origin Replication Restriction Sites Q

Features Vectors Plasmids Must Selectable Marker Origin Replication Restriction Sites Q

In contrast plasmids utilized in the lab are usually artificial and designed to introduce foreign DNA into another cell. Minimally [more]

PUC19 Vector Cloning: Free Transparent Background Png Clipart Mkoii

Free Transparent Background Png Clipart Mkoii

Peque 0003 Estrellitas Sabris Lara ilgraphicration transparent background PNG clipart size 252x212px filesize 38.1KB. Estrellita tr [more]

PUC19 Vector Cloning: Outline Of Psabr Construction The Psabr Plasmid Was Constructed By Substituting Afig

Outline Of Psabr Construction The Psabr Plasmid Was Constructed By Substituting Afig

Plasmid vector construction is an essential step for molecular microbiology yet often a time-consuming process. Manipulation of the fungal genome t [more]

PUC19 Vector Cloning: Dna Cloning Goal Is To Generate Large Amounts Of Pure Dna That Can Be Manipulated And Studied

Dna Cloning Goal Is To Generate Large Amounts Of Pure Dna That Can Be Manipulated And Studied

Definition purpose and basic steps of DNA cloning. Definition purpose and basic steps of DNA cloning. If youre seeing this message [more]

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